Molecular diagnosis for the detection of human metapneumovirus from viral isolates in pediatric patients with acute respiratory infection
Keywords:
Human metapneumovirus, acute respiratory infection, viral isolate, fusogenic, viral variantsAbstract
In Mexico the molecular epidemiology and viral etiology of acute respiratory infections are
very limited; there are no data about the frequency and circulation patterns of different
seasonal human Metapneumovirus. Therefore, we design a molecular technique to detect
and identify human Metapneumovirus. A total of 118 clinical samples from pediatric pa-
tients with low respiratory tract infections were isolated and classified by their fusogenic
activity as fusogenic and non fusogenic isolates. Moreover, fusogenic and non fusogenic
isolates were human Metapneumovirus confirmed by direct immunofluorescence. The
human metapneumovirus positive isolates (42/118) were confirmed by RT-PCR using
oligonucleotides designed in our laboratory to amplify the viral genes N (Nucleocapsid)
and/or L (RNA polimerase). We demonstrated by RT-PCR that human Metapneumovirus infection was detected in 100% of the viral isolates previously confirmed as positive by direct immunofluorescence assay (42/118). Alternatively, from the negative isolates by direct immunofluorescence (17), we were able to identify seven isolates as human respiratory syncytial virus using our designed RT-PCR.
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Copyright (c) 2016 Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra
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