Fundamentals of the polymerase chain reaction (PCR) and real-time PCR
Keywords:
PCR, Taq DNA Polymerase, Real Time PCR, SYBR Green, TaqmanAbstract
After three decades of discovering utility of polymerase chain reaction, its methodol-
ogy has become one of the most employed tools for the study of nucleic acids. This is a
top sensitive, highly reproductible, very efficient and short time consuming technology
that allows to obtain reliable and easy to evaluate results. Objectives of this paper deal
with fundamentals of both techniques standard polymerase chain reaction as well as the
polymerase chain reaction on real time. Results produced by standard polymerase chain
reaction are qualitative, while those produced by polymerase chain reaction on real time
are quantitative.
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