Comparison of the MicroScan WalkAway and VITEK 2 Compact systems for the identification and susceptibility of clinical Gram-positive and Gram-negative bacteria

Authors

  • Melissa Hernández-Durán Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • Luis E López-Jácome Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • Claudia A Colín-Castro Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • Guillermo Cerón-González Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • Silvestre Ortega-Peña Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • E Samuel Vanegas-Rodríguez Laboratorio de Infectología, Centro Nacional de Investiga-ción y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • Jaime A Mondragón-Eguiluz Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.
  • Rafael Franco-Cendejas Laboratorio de Infectología, Centro Nacional de Investigación y Atención a Quemados (CENIAQ), Instituto Nacional de Rehabilitación (INR), Mexico City, Mexico.

Keywords:

Bacteria, susceptibility antimicrobial test, accuracy, biochemistry/methods

Abstract

Introduction: The accuracy and fastness in bacterial identification and antimicrobial susceptibility are essentials in the management of the hospitalized patients with infectious diseases. Methodology: This study compares the usefulness of the semi-automated VITEK 2® Compact system vs. against the MicroScan WalkAway® SI system for bacterial identification and antimicrobial susceptibility. We included 54 bacterial strains isolated from hospitalized patients, 20 were Gram-positive cocci, 34 Gram-negative rods and 13 reference strains. Results: Of these strains, 89.5% were successfully identified at the species level by both systems. Concordance in susceptibility was 90.2% for Gram-negative and 96.3% for Gram-positive bacteria. Median delay time in obtaining the results of susceptibility testing was 6.5 h for VITEK 2 and 12.5 h for MicroScan. The MicroScan system presented a longer delay in obtaining results and greater difficulty in the correct identification of Gram-negative bacteria, Conclusions: Identification systems are a necessary tool in microbiological laboratories. Prompt and correct identification of clinical isolates aids in appropriate antimicrobial treatment.

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Published

2024-08-13

How to Cite

1.
Hernández-Durán M, López-Jácome LE, Colín-Castro CA, Cerón-González G, Ortega-Peña S, Vanegas-Rodríguez ES, et al. Comparison of the MicroScan WalkAway and VITEK 2 Compact systems for the identification and susceptibility of clinical Gram-positive and Gram-negative bacteria. Invest. Discapacidad [Internet]. 2024 Aug. 13 [cited 2024 Dec. 29];6(3):105-14. Available from: https://dsm.inr.gob.mx/indiscap/index.php/INDISCAP/article/view/325

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