Analysis of the giant cell tumor cell line of bone Tib 223 on the effect of quercetin.
Keywords:
cancer, Giant Cell Tumor of Bone, quercertin, flavonoid, apoptosisAbstract
Uncontrolled and inappropriate cell growth in the body is known as cancer. Giant Cell Tumor of Bone (GCTB) is a neoplasm characterized by aggressive behavior that, upon metastasis, manifests particularly in the lungs. The objective of this study was to evaluate the apoptotic effects of quercetin, a natural flavonoid, on the GCTB cell line (TIB223). These cells were treated with two distinct concentrations of quercetin. Flow cytometry analysis was performed, through which we observed a significant increase in caspase-3 gene expression and a decrease in PCNA gene levels. Following this analysis, qPCR was conducted to evaluate changes in the expression of genes regulating apoptosis (caspase-3) and proliferation (PCNA) after treatment. Flow cytometry results demonstrated decreased PCNA levels, indicating enhanced apoptosis and reduced proliferation, which suggests that quercetin effectively induces apoptotic pathways in GCTB cells. These findings provide insight into the molecular mechanisms underlying quercetin's anticancer activity, demonstrating its potential as a therapeutic agent for metastatic GCTB cells. We conclude that alternative therapies such as chemotherapy, radiotherapy, hormone therapy, or the use of the flavonoid quercetin show promise for treating various types of cancer, including bone, pancreatic, breast, and lung cancers.
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