Antioxidant role of polydatin in an in vitro gout model
Keywords:
Polydatin, monosodium urate crystals, gout, reactive oxygen species, antioxidantAbstract
Polydatin (POLY) is a polyphenolic compound found in the skin of the grapes, and in recent years their antioxidant and anti-inflammatory effects have been evaluated systemically, as well as chronic degenerative diseases (cardiovascular, diabetes, etc.). The gout, an arthropathy generated by the deposition of monosodium urate crystals (MSU) within joints and periarticular tissues, is characterized by an oxidant and inflammatory state mediated by synoviocytes, monocytes and neutrophils. Therefore, the aim of this study was to evaluate the effect of POLY in an in vitro model of oxidative stress induced by MSU. Pretreated monocytes with and without POLY, were exposed to MSU for 24 hours and intracellular production of reactive oxygen species (ROS) were evaluate between the two study groups and was compared with a group of unstimulated monocytes (control). The results show that monocytes exposed to MSU for 24 h increased ROS production (RFU 5.71) (p < 0.0001) compared to the control (1.00 RFU), promoting cellular oxidation condition; however, stimulated monocytes MSU but pretreated with POLY at concentrations of 10, 100 and 200 μM ROS, ROS production decreased to 1.95, 1.41 and 0.93 RFU, respectively (p < 0.0001). POLY participates as antioxidant in our in vitro model, which makes possible a molecule with therapeutic potential; however are needed further studies to better understand the antioxidant effects POLY in order to identify new therapeutic targets in this disease.
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Copyright (c) 2017 Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra
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© Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra under a Creative Commons Attribution 4.0 International (CC BY 4.0) license which allows to reproduce and modify the content if appropiate recognition to the original source is given.
