Western blot: a tool in the biomedical field
Keywords:
Western blot, immunodetection, electrophoresis, protein, immunoblot, antibodyAbstract
The Western blot technique, also called immunoblotting, is a high-sensitivity and semi-quantitative molecular technique that allows the analysis of a specific protein or protein profile. This is a technique for separating proteins according to their charge and molecular weight in polyacrylamide gels and their subsequent transference to a solid membrane, in which a specific antibody detects the protein. Four decades after its creation, the Western blot technique has gained an essential role in the diagnosis of various medical conditions, as well as an everyday tool in biomedical research.
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