Molecular diagnosis for the detection of human metapneumovirus from viral isolates in pediatric patients with acute respiratory infection
Keywords:
Human metapneumovirus, acute respiratory infection, viral isolate, fusogenic, viral variantsAbstract
In Mexico the molecular epidemiology and viral etiology of acute respiratory infections are very limited; there are no data about the frequency and circulation patterns of different seasonal human Metapneumovirus. Therefore, we design a molecular technique to detect and identify human Metapneumovirus. A total of 118 clinical samples from pediatric patients with low respiratory tract infections were isolated and classified by their fusogenic activity as fusogenic and non fusogenic isolates. Moreover, fusogenic and non fusogenic isolates were human Metapneumovirus confirmed by direct immunofluorescence. The human metapneumovirus positive isolates (42/118) were confirmed by RT-PCR using oligonucleotides designed in our laboratory to amplify the viral genes N (Nucleocapsid) and/or L (RNA polimerase). We demonstrated by RT-PCR that human Metapneumovirus infection was detected in 100% of the viral isolates previously confirmed as positive by direct immunofluorescence assay (42/118). Alternatively, from the negative isolates by direct immunofluorescence (17), we were able to identify seven isolates as human respiratory syncytial virus using our designed RT-PCR.
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Copyright (c) 2016 Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra
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© Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra under a Creative Commons Attribution 4.0 International (CC BY 4.0) license which allows to reproduce and modify the content if appropiate recognition to the original source is given.
